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Shanghai Aladdin Bio-Chem
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Matrix Remodeling-Associated Protein 8 as a Novel Indicator Contributing to Glioma Immune Response by Regulating Ferroptosis
doi: 10.3389/fimmu.2022.834595
Figure Lengend Snippet: MXRA8 regulates ferroptosis in glioma. (A) The correlation between expression of MXRA8 and ferroptosis-related proteins including NCOA4 and FTH1. (B) NCOA4 and FTH1 were examined by Western blot in U251 and T98G cells transfected with siNC or siMXRA8. (C, D) Fe 2+ and MDA levels were detected in U251 and T98G cells transfected with siNC or siMXRA8. (E) Cell viability was measured in siNC- or siMXRA8-transfected cells after pretreatment with RSL3 (5 μM) and subsequent Fer-1 (20 μM). (F, G) Fe 2+ and MDA levels detected in siNC- or siMXRA8-transfected cells pretreated with RSL3 (5 μM) and Fer-1 (20 μM) for 24 h, successively. (H) After treatment of 5 μM of RSL3 in U251 and T98G cells, Western blot assay were performed to examine the protein expression of MXRA8. * p < 0.05; ** p < 0.01.
Article Snippet: Ras-selective lethal small molecule 3 (RSL3) and
Techniques: Expressing, Western Blot, Transfection
Journal: Frontiers in Immunology
Article Title: Matrix Remodeling-Associated Protein 8 as a Novel Indicator Contributing to Glioma Immune Response by Regulating Ferroptosis
doi: 10.3389/fimmu.2022.834595
Figure Lengend Snippet: The effects of MXRA8 on M2 macrophage infiltration in vitro by mediating ferroptosis. (A) The correlation coefficient between MXRA8 and classical phenotype markers of macrophages in the GEO datasets. (B) The expression level of MXRA8 and a M2 macrophage phenotype marker (CSF1R) in GBM and normal brain specimens determined by IHC analysis. (C) Infiltration of M2 macrophages in transfected siNC or siMXRA8 cells (U251 and T98G) after pretreatment with RSL3 (5 μM) and subsequent Fer-1 (20 μM). *** p < 0.001.
Article Snippet: Ras-selective lethal small molecule 3 (RSL3) and
Techniques: In Vitro, Expressing, Marker, Transfection